abstract：AIMS:Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and if the morphological and biochemical features of PCD are conserved. METHODS AND RESULTS:Using TUNEL labelling, fluorescence and light microscopy and DNA gel electrophoresis, we found that cell death with features simil...

journal_title：Journal of applied microbiology

authors： Ning SB,Guo HL,Wang L,Song YC

更新日期：2002-01-01 00:00:00

abstract：AIMS:This study was performed to analyse the molecular characteristics of genes encoding for the major virulence factors in Bacillus anthracis vaccine strain 'Carbosap' compared with the wild B. anthracis strain, to evaluate the basis of attenuation. METHODS AND RESULTS:The molecular characteristics of the B. anthraci...

journal_title：Journal of applied microbiology

authors： Adone R,Pasquali P,La Rosa G,Marianelli C,Muscillo M,Fasanella A,Francia M,Ciuchini F

更新日期：2002-01-01 00:00:00

abstract：:Recent advances in DNA sequencing technology have made it possible to elucidate the entire genomes of pathogenic bacteria, and advancements in bioinformatic tools have driven comparative studies of these genome sequences. These evaluations are dramatically increasing our ability to make valid considerations of the lim...

journal_title：Journal of applied microbiology

authors： McDevitt D,Payne DJ,Holmes DJ,Rosenberg M

更新日期：2002-01-01 00:00:00

abstract：AIMS:The aims of the project were threefold: to survey the use of disinfectants in the UK food industry; to assess the product and environmental microflora of selected food factories for the persistence of Listeria monocytogenes and Escherichia coli; and to determine the disinfectant resistance of any persistent strain...

journal_title：Journal of applied microbiology

authors： Holah JT,Taylor JH,Dawson DJ,Hall KE

更新日期：2002-01-01 00:00:00

abstract：AIMS:The aims of the study were to characterize the O91 O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91. METHODS AND RESULTS:The published primers complementary to JUMPstart and gnd gene, the conserved fla...

journal_title：Journal of applied microbiology

authors： Perelle S,Dilasser F,Grout J,Fach P

更新日期：2002-01-01 00:00:00

abstract：AIM:To introduce the gfp gene encoding green fluorescent protein (GFP) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. METHODS AND RESULTS:Bradyrhizobium sp. strains M29 and GN7, which nodulate mungbean (Vigna radiata), were conjugated with Escherichia coli S17-1 carrying pla...

journal_title：Journal of applied microbiology

authors： Bhatia R,Dogra RC,Sharma PK

更新日期：2002-01-01 00:00:00

abstract：AIMS:The aims of this study were to evaluate the ability of exogenous Lactobacillus acidophilus strain NCFM to survive through the human gastro-intestinal (GI) tract, and to evaluate the selectivity of Rogosa SL medium for faecal lactobacilli. METHODS AND RESULTS:The composition of the faecal lactobacilli of 10 health...

journal_title：Journal of applied microbiology

authors： Sui J,Leighton S,Busta F,Brady L

更新日期：2002-01-01 00:00:00

abstract：AIMS:Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. METHODS AND RESULTS:Thirty-five Lactob...

journal_title：Journal of applied microbiology

authors： Pavlova SI,Kilic AO,Kilic SS,So JS,Nader-Macias ME,Simoes JA,Tao L

更新日期：2002-01-01 00:00:00

abstract：AIMS:To isolate, select, identify and assess the potential for the biodegradation of synthetic pyrethroids (SPs) in sheep dips. METHODS AND RESULTS:SP-degrading bacteria were isolated from a mixed soil sample consisting of garden soil and soils from farms where SPs had been used. The two largest in size were then iden...

journal_title：Journal of applied microbiology

authors： Grant RJ,Daniell TJ,Betts WB

更新日期：2002-01-01 00:00:00

abstract：AIMS:The aim of this study was to develop a method based on immunomagnetic capture and polymerase chain reaction (IC-PCR assay) for detection of Cryptosporidium parvum and Giardia intestinalis in sewage sludge. METHODS AND RESULTS:The detection limit of the IC-PCR assay for both organisms was 625 oocysts and cysts ml(...

journal_title：Journal of applied microbiology

authors： Rimhanen-Finne R,Ronkainen P,Hänninen ML

更新日期：2001-12-01 00:00:00

abstract：AIMS:To investigate the effects of whey retentate from Bifidobacteria fermented milk. METHODS AND RESULTS:The simulator of the human intestinal microbial ecosystem (SHIME) was used. The composition of the microbiota and its metabolic activities were analysed. Changes in the microbial composition became apparent within...

journal_title：Journal of applied microbiology

authors： Meddah AT,Yazourh A,Desmet I,Risbourg B,Verstraete W,Romond MB

更新日期：2001-12-01 00:00:00

abstract：AIMS:Evaluation of the efficiency of peracetic acid in the disinfection of wastewater in a large treatment plant. METHODS AND RESULTS:Over a period of 18 months 30 sample collections were made, each consisting of three samples taken from: raw incoming sewage, secondary effluent (after 10-12 h) and secondary effluent d...

journal_title：Journal of applied microbiology

authors： Stampi S,De Luca G,Zanetti F

更新日期：2001-11-01 00:00:00

abstract：AIMS:The study of a protease secreted by Candida caseinolytica for use in future industrial applications. METHODS AND RESULTS:Growth of Candida caseinolytica on a medium containing milk induced a rapid production of an extracellular enzyme able to hydrolyse casein. The crude extract was applied to both Sephacryl S-200...

journal_title：Journal of applied microbiology

authors： Poza M,de Miguel T,Sieiro C,Villa TG

更新日期：2001-11-01 00:00:00

abstract：AIMS:To characterize a bacteriocin-like factor from Bacillus licheniformis 26 L-10/3RA isolated from buffalo rumen. METHODS AND RESULTS:The culture supernatant exhibited the antibacterial activity against a number of indicator organisms in a cut-well agar assay under anaerobic conditions. The inhibitory component was ...

journal_title：Journal of applied microbiology

authors： Pattnaik P,Kaushik JK,Grover S,Batish VK

更新日期：2001-10-01 00:00:00

abstract：AIMS:To determine whether isolates of Listeria monocytogenes differ in their ability to adsorb and form biofilms on a food-grade stainless steel surface. METHODS AND RESULTS:Strains were assessed for their ability to adsorb to a test surface over a short time period. Although some differences in numbers of bound cells...

journal_title：Journal of applied microbiology

authors： Kalmokoff ML,Austin JW,Wan XD,Sanders G,Banerjee S,Farber JM

更新日期：2001-10-01 00:00:00

abstract：AIMS:The minimum inhibitory concentration (MIC) of oregano essential oil (OEO) and two of its principle components, i.e. thymol and carvacrol, against Pseudomonas aeruginosa and Staphylococcus aureus was assessed by using an innovative technique. The mechanism of action of the above substances was also investigated. M...

journal_title：Journal of applied microbiology

authors： Lambert RJ,Skandamis PN,Coote PJ,Nychas GJ

更新日期：2001-09-01 00:00:00

abstract：AIMS:The purpose of this study was to compare the efficacy, in terms of bacterial biofilm penetration and killing, of alkaline hypochlorite (pH 11) and chlorosulfamate (pH 5.5) formulations. METHODS AND RESULTS:Two species biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown by flowing a dilute medi...

journal_title：Journal of applied microbiology

authors： Stewart PS,Rayner J,Roe F,Rees WM

更新日期：2001-09-01 00:00:00

abstract：AIMS:Campylobacter contamination in French chicken production from the farm to the consumer was determined using a PCR assay for bacteria detection and identification. METHODS AND RESULTS:Samples were bird droppings from poultry houses, neck skins, livers, hearts, gizzards, wings, legs and escalopes from slaughterhous...

journal_title：Journal of applied microbiology

authors： Denis M,Refrégier-Petton J,Laisney MJ,Ermel G,Salvat G

更新日期：2001-08-01 00:00:00

abstract：AIMS:To achieve reliable detection of methicillin resistance in clinical isolates of coagulase-negative staphylococci. METHODS AND RESULTS:Strains (105) were evaluated by normatized antimicrobial susceptibility methods, and for the presence of the methicillin resistance-determining mecA gene, using the polymerase chai...

journal_title：Journal of applied microbiology

authors： Bogado I,Sutich E,Krapp A,Marchiaro P,Marzi M,Putero J,Carrillo N

更新日期：2001-08-01 00:00:00

abstract：AIMS:The shelf-life of refrigerated catfish fillets was determined at 2 degrees C, to simulate retail conditions, using two types of packaging materials, vacuum packing (VP) and oxygen permeable packaging (OPP). METHODS AND RESULTS:Representative samples (n=5) from both types of packaging methods were drawn at random ...

journal_title：Journal of applied microbiology

authors： Anelich LE,Hoffman LC,Swanepoel MJ

更新日期：2001-07-01 00:00:00

abstract：AIMS:The purpose of this study was to develop a laboratory biofilm growth reactor system that simulated the toilet bowl environment and which could be used for biocide efficacy testing. METHODS AND RESULTS:A microbial biofilm reactor system incorporating intermittent flow and nutrient provision was designed. The react...

journal_title：Journal of applied microbiology

authors： Pitts B,Willse A,McFeters GA,Hamilton MA,Zelver N,Stewart PS

更新日期：2001-07-01 00:00:00

abstract：AIMS:This project aimed to investigate the mechanism of attachment of the vegetative cells and spores of thermophilic bacilli to stainless steel with a view to devising strategies to limit biofilm development and survival. METHODS AND RESULTS:Spores and vegetative cells of bacterial isolates were exposed to protein de...

journal_title：Journal of applied microbiology

authors： Parkar SG,Flint SH,Palmer JS,Brooks JD

更新日期：2001-06-01 00:00:00

abstract：AIMS:This study investigated whether the higher incidence of recovery from meat of Listeria innocua compared with L. monocytogenes could be due to the laboratory media used, leading to an artificially lower detection of the pathogenic species, L. monocytogenes. METHODS AND RESULTS:Minced beef was inoculated with L. mo...

journal_title：Journal of applied microbiology

authors： Duffy G,Walsh D,Sheridan JJ,Logue CM,Harrington D,Blair IS,McDowell DA

更新日期：2001-06-01 00:00:00

abstract：AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...

journal_title：Journal of applied microbiology

authors： Abreu C,Jurgens G,De Marco P,Saano A,Bordalo AA

更新日期：2001-05-01 00:00:00

abstract：AIMS:To study the effects of temperature, pH and yeast extract (YE) concentration on growth and exopolysaccharide (EPS) production by Propionibacterium acidi-propionici DSM 4900 cultivated on milk microfiltrate. METHODS AND RESULTS:A multifactorial approach using a Response Surface Methodology (RSM) was followed. The ...

journal_title：Journal of applied microbiology

authors： Gorret N,Maubois JL,Engasser JM,Ghoul M

更新日期：2001-05-01 00:00:00

abstract：AIMS:Nutritional requirements of Lactobacillus fermentum Ogi E1 were studied in order to define a simplified fermentation medium. METHODS AND RESULTS:When grown with MRS-medium in 2l bioreactors, a biphasic pattern of growth and metabolite production was observed. Study of nutritional requirements resulted in a simpli...

journal_title：Journal of applied microbiology

authors： Calderon M,Loiseau G,Guyot JP

更新日期：2001-04-01 00:00:00

abstract：AIMS:The aim of the study was to assess the relationships between the remaining shelf-life (RSL) of cold-smoked salmon and various microbiological and physico-chemical parameters, using a multivariate data analysis in the form of stepwise forward multiple regression. METHODS AND RESULTS:Thirteen batches of French cold...

journal_title：Journal of applied microbiology

authors： Leroi F,Joffraud JJ,Chevalier F,Cardinal M

更新日期：2001-04-01 00:00:00

abstract：AIMS:The chitinolytic activity of an actinomycete, isolated from a tropical acidic ferrasol (FAO) under cerrado (savanna) vegetation, is reported. METHODS AND RESULTS:Selection of the strain was based on spot inoculation on solid colloidal chitin medium. The use of chemotaxonomic, morphological and physiological proce...

journal_title：Journal of applied microbiology

authors： Gomes RC,Sêmedo LT,Soares RM,Linhares LF,Ulhoa CJ,Alviano CS,Coelho RR

更新日期：2001-04-01 00:00:00

abstract：AIMS:Yersinia enterocolitica causes several syndromes in humans. The most common presentation is enterocolitis in children, presenting as fever and diarrhoea. A Y. enterocolitica multiple strain infection in twin infants was investigated. METHODS AND RESULTS:One isolate was recovered from one patient and two morpholog...

journal_title：Journal of applied microbiology

authors： Gray JT,WaKabongo M,Campos FE,Diallo AA,Tyndal C,Tucker CA

更新日期：2001-03-01 00:00:00

abstract：AIMS:To validate an in vitro model for the analysis of physiological and ecological responses to sugar challenge in bacterial populations, and subsequent changes in enamel mineralization. METHODS AND RESULTS:A seven-organism bacterial consortium was grown in a biofilm mode on enamel and hydroxyapatite (HA) surfaces in...

journal_title：Journal of applied microbiology

authors： Hodgson RJ,Lynch RJ,Watson GK,Labarbe R,Treloar R,Allison C

更新日期：2001-03-01 00:00:00

abstract：AIMS:A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. METHODS AND RESULTS:The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of time. The microcolonie...

journal_title：Journal of applied microbiology

authors： Perry-O'Keefe H,Stender H,Broomer A,Oliveira K,Coull J,Hyldig-Nielsen JJ

更新日期：2001-02-01 00:00:00

abstract：AIMS:To employ an in vitro screening regime to select a probiotic Bifidobacterium strain to complement resistant starch (Hi-maizetrade mark) in a synbiotic yoghurt. METHODS AND RESULTS:Of 40 Bifidobacterium isolates examined, only B. lactis Laftitrade mark B94 possessed all of the required characteristics. This isolat...

journal_title：Journal of applied microbiology

authors： Crittenden RG,Morris LF,Harvey ML,Tran LT,Mitchell HL,Playne MJ

更新日期：2001-02-01 00:00:00

abstract：AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...

journal_title：Journal of applied microbiology

authors： Barbe JC,De Revel G,Joyeux A,Bertrand A,Lonvaud-Funel A

更新日期：2001-01-01 00:00:00

abstract：AIMS:To determine the 16S rRNA gene fingerprints of Bacillus thuringiensis strains to reveal phylogenetic relationships among them. METHODS AND RESULTS:Using 16S rRNA gene restriction fragment length polymorphisms generated by HindIII and EcoRI, 86 Bacillus thuringiensis strains were classified. This includes 80 B. th...

journal_title：Journal of applied microbiology

authors： Joung KB,Côté JC

更新日期：2001-01-01 00:00:00

abstract：AIM:The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. METHODS AND RESULTS:API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from...

journal_title：Journal of applied microbiology

authors： Nigatu A

更新日期：2000-12-01 00:00:00

abstract：:Microbial succession during a laboratory-scale composting process of garbage was analysed by denaturing gradient gel electrophoresis (DGGE) combined with measurement of physicochemical parameters such as temperature, pH, organic acids, total dissolved organic carbon and water-soluble humic substance. From the temperat...

journal_title：Journal of applied microbiology

authors： Ishii K,Fukui M,Takii S

更新日期：2000-11-01 00:00:00

abstract：:The surface hydrophobicity, viability and biocontrol ability of Penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. A phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydro...

journal_title：Journal of applied microbiology

authors： Pascual S,De Cal A,Magan N,Melgarejo P

更新日期：2000-11-01 00:00:00

abstract：:A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...

journal_title：Journal of applied microbiology

authors： Hartikainen T,Ruuskanen J,Räty K,Von Wright A,Martikainen PJ

更新日期：2000-10-01 00:00:00

abstract：:In this study, three flor strains of Saccharomyces cerevisiae were genetically characterized. They were isolated from biofilms on Sardinian sherry-like wines produced at family-run wineries where pure cultures of yeasts were not used. The study aimed to investigate the life cycle of these naturally-occurring flor stra...

journal_title：Journal of applied microbiology

authors： Budroni M,Giordano G,Pinna G,Farris GA

更新日期：2000-10-01 00:00:00

abstract：:A 210-min-test for the enumeration of Escherichia coli in drinking water is described, based on solid phase cytometry (SPC) and a two-step enzymatic procedure for fluorescence labelling of single cells and small microcolonies. The test involves membrane filtration through a 25-mm black polyester filter, induction of b...

journal_title：Journal of applied microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：2000-09-01 00:00:00